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Welcome to  SASSY GLASS!

We offer the best in Jeweled Wine Glass Charms, Unique Bottle Stoppers and Rhinestone Embellished  Glasses

 Planning a party? Make A Sassy Glass your first stop! Sassy Glass charms ,glasses and stoppers always make a great gift!   Perfect for Bachelorette parties, birthday's,bridal showers or any occasion where you want to entertain with some "Sass"

    hub sciencedirect scopus applications register login login go to scival suite username: password: remember me | not registered? Forgotten your username or password? Go to athens / institution login remote access activation home publications search my settings my alerts shopping cart help export citation purchase more options... Email article signed up for journal alerts [remove] alert me about new articles in this journal your selection(s) could not be saved due to an internal error. Please try again. Search     all fields     author advanced search     journal/book title     volume   issue   page search tips article outline is loading... Javascript required for article outline small ruminant research volume hundred, issues 2–3, october 2011, pages 169–176 expression of growth and differentiation factor 9 (gdf-9) and its effect on the in vitro culture of caprine preantral ovarian follicles a. viagra for sale secure canadian pharmacy online cheap viagra viagra for sale cheap viagra viagra without a doctor prescription cheap viagra wholesale viagra without a doctor prescription viagra without a doctor prescription buy cheap viagra P. Almeida a , , , m. V. A. Saraiva a , v. R. Araújo a , d. M. Magalhães a , a. B. G. Duarte a , i. M. A. Frota b , c. A. P. Lopes a , c. C. Campello a , j. R. V. Silva b , j. R. Figueiredo a a laboratory of manipulation of oocytes and preantral follicles (lamofopa), faculty of veterinary, state university of ceará, fortaleza, ce, 60740-903, brazil b biotechnology nucleus of sobral (nubis), federal university of ceará, sobral, ce, 62042-280, brazil received 2 june 2010 revised 23 may 2011 accepted 7 june 2011 available online 1 july 2011 how to cite or link using doi permissions & reprints view full text purchase $31. 50 sign up for read-only pdf 1. Introduction 2. Materials and methods 3. Results 4. Discussion references abstract this study examined the expression of growth and differentiation factor 9 (gdf-9) in caprine ovarian follicles, and the effect of gdf-9 with or without fsh on the in vitro culture of preantral follicles. To evaluate the expression of gdf-9 in experiment 1, follicles were recovered from 32 goat ovaries and the total rna isolated and transcribed for real-time polymerase chain reaction (pcr). Experiments 2 and 3 each used a further 32 goat ovaries to provide preantral follicles of ≥150 î¼m. These follicles were isolated and cultured individually in 100 î¼l drops. In each experiment at least 45 follicles were used per treatment. Every 6 days, follicles were evaluated for viability, antrum formation and growth rate. At the end of the culture period, oocytes were submitted to in vitro maturation (ivm), viability tests and chromatin evaluation. In experiment 2, follicles were cultured in a basal medium (control) or this medium supplemented with gdf-9 at a concentration of 100 ng/ml (gdf-9 c) or 200 ng/ml (gdf-9 cardinal). The same media were used in experiment 3, supplemented with recombinant fsh at a level of 100 ng/ml from day 0, 500 ng/ml from day. We are currently adding more rhinestone embellished glasses! 

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